Parasporin, a Human Leukemic Cell-Recognizing Parasporal Protein of Bacillus thuringiensis
نویسندگان
چکیده
منابع مشابه
Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis.
An unusual property, human leukemic cell-recognizing activity, associated with parasporal inclusions of a noninsecticidal Bacillus thuringiensis soil isolate was investigated, and a protein (named parasporin in this study) responsible for the activity was cloned. The parasporin, encoded by a gene 2,169 bp long, was a polypeptide of 723 amino acid residues with a predicted molecular weight of 81...
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Parasporin (PS) is a collection of genealogically heterogeneous Cry proteins synthesized in Bacillus thuringiensis. A prominent feature commonly associated with PS proteins is the strong cytocidal activity preferential for human cancer cells of various origins. The proteins exhibit cytocidal activities only when digested with proteases. Currently, this protein group is classified into four fami...
متن کاملInvestigation of Cytocidal Activity of Bacillus Thuringiensis Parasporal Toxin on CCRF-CEM Cell Line
Background & Objective: Parasporin is a parasporal protein of Bacillus thuringiensis and exhibits special cytocidal activity against human cancer cells. Similar to other insecticidal Bacillus thuringiensis crystal toxins, parasporin shows target specificity and damages the cellular membrane. In this study, different strains of Bacillus thuringiensis isolated from various regions of Iran and the...
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Parasporin is the cytocidal protein present in the parasporal inclusion of the non-insecticidal Bacillus thuringiensis strains, which has no hemolytic activity but has cytocidal activities, preferentially killing cancer cells. In this study, we characterized a cytocidal protein that belongs to this category, which was designated parasporin-5 (PS5). PS5 was purified from B. thuringiensis serovar...
متن کاملIdentification of parasporin genes in Vietnamese isolates of Bacillus thuringiensis.
Four genes encoding parasporins, cytotoxins preferentially killing human cancer cells in vitro, were isolated from four Vietnamese strains of Bacillus thuringiensis. Nucleotide sequence analysis revealed that: (1) three genes fall into the two known classes, ps1Aa and ps1Ab, and (2) another one belongs to ps1Ac, a novel gene class established in this study. Upon proteolytic activation, paraspor...
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ژورنال
عنوان ژورنال: Clinical Diagnostic Laboratory Immunology
سال: 2000
ISSN: 1071-412X,1098-6588
DOI: 10.1128/cdli.7.4.625-634.2000